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The screening services cover the costs of compounds, multi-well plates, general buffers, and luminescence detection reagents (CellTiterGlo, BrightGlo, and NanoGlo).
Qualified Assays for Screening
Protein-based biochemical assays (fluorescence- or luminescence-based assays)
These screens use purified proteins and substrates in buffered solutions to produce an optical (fluorescent, luminescent, etc.) readout that monitors enzymatic or binding activity. In these types of screens, small compound inhibitors are ranked by the SIGNALS, referring to the function of targeted protein.
Examples:
FRET substrates for proteases
Fluorophore-tagged glycosides for glycosidase inhibitors
Fluorophore-tagged ligands for protein binding inhibitors
Luciferase fragment-tagged proteins to measure the dimerization
Luciferase fragment-tagged proteins to measure the protein-protein interaction
HDAC-Glo for HDACs
NADP-Glo for NADP-utilizing enzymes
Cell-based assays (Luciferase-based signal detection or Image-based analysis)
These screens can be macromolecular or small-molecule screens that utilize cells plated in 96 or 384 or 1536 well plates to produce visual phenotypic or signal changes in the cells. The measurement can be a signal- or image-based readouts.
Examples:
Cytotoxicity evaluation using CellTiterGlo
Promoter-driven expression of luciferase (firefly, NanoLuc, Lucia)
Promoter-driven expression of fluorescent proteins
Luciferase fragment-tagged proteins to measure dimerization or protein binding
Fluorescent protein-tagged proteins to measure expression of proteins
Fluorescent protein-tagged proteins to monitor localization
Library for Screening
Pilot (2K)
FDA-approved library from TargetMol. The library contains ~1,200 FDA- approved drugs and 800 clinical compounds. Ideal for a pilot test of the assay system.
The cost is NTD$20,000 for Taiwan researchers. Additional options for cell culture are available.
Researchers who are not in Taiwan can request a quote.
Probe Set (9K)
The library contains ~3,000 approved drugs, clinical compounds, and randomized compounds with annotated activities. Ideal for a starting set of probing potential hits for the target of interest.
The cost is NTD$60,000 for Taiwan researchers. Additional options for cell culture are available.
Researchers who are not in Taiwan can request a quote.
Traditional Chinese Medicine (2K)
The library contains 2,000 pure compounds commonly found in Traditional Chinese Medicine.
The cost is NTD$30,000 for Taiwan researchers. Additional options for cell culture are available.
Researchers who are not in Taiwan can request a quote.
Bioactive Compounds (15K)
The library contains known bioactive compounds, such as kinase inhibitors, protease inhibitors, etc.
The cost is NTD$120,000 for Taiwan researchers. Additional options for cell culture are available.
Researchers who are not in Taiwan can request a quote.
Natural Products (50 K)
The library contains natural products and their derivatives from InterBioScreen, Inc.
Discovery Diversity Set (60 K)
The library contains structurally diverse compounds from Enamine (Discovery Diversity Set).
The cost is NTD$200,000 for Taiwan researchers. Additional options for cell culture are available. Researchers who are not in Taiwan can request a quote.
Representative Compounds (80 K)
The library contains representative compounds from GRC collection.
Customized library
Cannot find the library of interest? Have your own compound list? Create your own library from GRC collection!
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